Investigating the Effect of Temperature on the Movement of Pigment thro

Investigating the Effect of Temperature on the Movement of Pigment by Cell MembranesINTRODUCTIONThe aim of experiment is to prove that temperature effects on aproteins in cell tissue layers so that pigment can light up through themHYPOTHESISOn senior high temperatures proteins in tissue layers denature so there is nobarrier to nix the passage of large molecules hypothetical BACKGROUNDCertain chemicals and treatments, such as ethanol or hightemperatures, can destroy the partial permeability of cell membrane.The membranes are pipe down present but behave as if holes have beenpunched through them and they no longer provide barrier to the passageof large molecules such as sucrose. High temperatures and alcoholsdenature membrane proteins and increase fluidity of membrane lipidsalcohols at high cin one casentrations can also dissolve lipids.In beet cells the reddened anthocyanin pigment occurs in the vacuoles.Each vacuole is surrounded by a tonoplast membrane and outside of it,the cytoplasm is surrounded by the plasma membrane. On highertemperatures proteins loose their ability of control the transportthrough cell so any large molecule can pass trough.APPARATUS AND MATERIALS attempt vacuum tube rack with 10 test tubes Graduated syringe Scalpel dock borer Stop watch Forceps Colorimeter Tap root of beetroot Distilled peeingSAFETY Carefully use of profounds and services cork borer and scalpel to interrupt injuries by cutting Use bunsen burner with great precaution to prohibit burn or lightingmaterials Water in beaker is very voluptuous so watch out that you dont spill it onyou to prevent burns Watch out that you dont break any glass apparatus to preventcuttingMETHOD Using a cork borer and scalpel restrain 10 cylinders of beetroot of 5 cmlength. Heat 200 cm3 of water up to 85 C in 250 cm3 beaker (make sure thatthermometer is in the water). go heating, with a syringe, put 10 ml of distilled water in 10test tubes and label them 85, 80, 75, 70, 65, 63, 6 0, 55, 50 and 45. When 85C temperature is reached remove the Bunsen burner and putone beetroot cylinder in the water and surrender it there for exactly oneminute. After one minute use forceps to post it out and put it in a testtube marked 85. Put some cold water in the beaker until 80 C is reached and t... ...tly ever-changing. In some moments changing would stop but thatisnt real sign that that is the right colour density becauseafter couple of moments changing would start again. Solution is to usemore sensitive equipment or to write down colorimeter checked because itcould be broken. Fifth problem is in benevolent nature. During the test we had a goodtime so occasionally we forgot to take the beetroot out for fewseconds longer than it should be. We didnt pay all assist toprofessor so we forgot to shake test tubes before we took the beetrootout. Also, I didnt take a paper with me during the colour test sowhen I got result, occasionally I would forget what it was while I wasg oing impale to my seat. Also there was some noise in the classroom so Icould misunderstand my partners in the experiment when they came withresult. To be sure in experiment results the best thing is to repeat it atleast once to increase the reliability of them. Now when I know thewhole affair of experiment, results in repetitive experiment shouldbe more accurate. Also this is good background to compare howdifferent concentrations of ethanol effect on the membranepermeability.